BIO 5406
McGilliard

Reading Assignment:

     Hadley, pp. 67-90.

     Witherspoon, L.  Immunoassay: The evolution of a revolution in disease diagnosis.  Modern
          Medicine 57(3): 68-72, 1989.
 

     Soulet, D., and Rivest, S.  Perspective:  How to make microarray, serial analysis of gene

          expression, and proteomic relevant to day-to-day endocrine problems and physiological

          systems.  Endocrinology 143: 1995-2001, 2002.

Objectives:

     After reviewing your lecture notes and reading assignment, you should be able to:

     1.  Describe and give an example of the classic technique of endocrinology.

          Lab -- 1/15

          Text -- pp. 67, 69-70

     2.  Describe the principles and uses of each of the following experimental techniques,

          with examples from endocrinology:
          a.  Bioassay.
          b.  Competitive protein binding assay.
          c.  Radioimmunoassay (RIA).
          d.  Enzyme-linked colorimetric immunoassay.
          e.  Immunocytochemistry.
 

          Lab/Lecture -- 1/15, 1/16
          Text -- pp. 71-77

          Figures -- 4.2, 4.3, 4.4, 8.18
          Witherspoon article

     3.  Give an example of the use of recombinant DNA technology in the advancement

          of endocrinology.

          Lecture -- 1/16

          Text -- pp. 85-86

          Figure -- 4.11

     4.  Discuss the use of transgenic and knock-out animals in endocrinology research.

          Lecture -- 1/16

          Text -- pp. 86-88

          Figure -- 4.12

     5.  Throughout the semester, be prepared to design experiments which demonstrate
          various properties of endocrine glands or principles of endocrinology.

     6.  Describe the contributions of Rosalyn Yalow and Solomon Berson to the field of
          endocrinology.
 

          Lab -- 1/15
          Text -- pg. 6
          1977 Nobel Press Release