LABORATORY:  THYROID FUNCTION

 

 

GO TO STATISTICAL ANALYSIS

 

 

DATA

 

 

Group A -- Control

 

Cage #

Body Weight (g)

Initial

Week 3

Change

1

27.4

29.2 1.8

1

28.9

29.8 0.9

1

30.2

30.8 0.6

2

29.1

29.5 0.4

2

29.4

30.3 0.9

2

32.0

31.3 -0.7

Mean

29.5

30.2 0.7

SD

 

   

 

 

Cage #

O2 Consumption in 5 min (ml)

Average 
O2Consumption 
(ml O2/min/kg)

Trial #1

Trial #2

Trial #3

1

13.4 15.3 -- 95

1

15.3 15.5 -- 103.4

1

12.4 13.0 -- 82.5

2

11 7.5 -- 62.7

2

6 9.5 -- 51.2

2

8 10.5 -- 59.1

Mean

 

 

 

75.7

SD

 

 

 

21.1

 

 

Group B -- Thyroxine

 

Cage #

Body Weight (g)

Initial

Week 3

Change

3

28.9

28.5 -0.4

3

31.8

29.0 -2.8

3

32.4

29.0 -3.4

4

28.0

28.5 0.5

4

28.2

27.6 -0.6

4

29.9

29.2 -0.7

Mean

29.9

28.6 -1.3

SD

 

   

 

 

Cage #

O2 Consumption in 5 min (ml)

Average 
O2Consumption 
(ml O2/min/kg)

Trial #1

Trial #2

Trial #3

3

15.7 12.2 -- 97.9

3

19.0 16.3 -- 121.7

3

14.8 18.3 -- 114.1

4

12.5 7 15 96.5

4

11.5 11 -- 81.5

4

13 11.5 -- 83.6

Mean

 

 

 

99.2

SD

 

 

 

16.1

 

 

Group C -- PTU

 

Cage #

Body Weight (g)

Initial

Week 3

Change

5

28.3

26.8 -1.5

5

28.9

28.9 0.0

5

31.1

29.6 -1.5

6

28.9

28.5 -0.4

6

29.0

30.5 1.5

6

30.2

32 1.8

Mean

29.4

29.4 0

SD

 

   

 

 

Cage #

O2 Consumption in 5 min (ml)

Average 
O2Consumption 
(ml O2/min/kg)

Trial #1

Trial #2

Trial #3

Trial #4

5

9.0 7.6 8.9 10.1 66.4

5

12.2 11.6 10.4 10.6 77.5

5

5.2 3.8 14.7 13.7 95.9

6

12.5 11 -- -- 82.5

6

15 14.5 -- -- 96.7

6

15 15 -- -- 93.8

Mean

 

 

 

  85.5

SD

 

 

 

  12.2

 

 

 

STATISTICAL ANALYSIS OF

OXYGEN CONSUMPTION DATA

 

 

ONE-WAY ANALYSIS OF VARIANCE

 

 

Source

df

Sum of 
Squares

Mean 
Square

F

P

Treatment

2

1681.63

840.82

2.96

0.08

Error

15

4265.00

284.33

Total

17

5946.63

 

Conclusion:

 

There was no significant difference between treatment means

(P < 0.05 is required for statistical significance).

 

 

 Pair-wise comparison of means using Dunnett's Test:

 

Critical mean differences:  P0.05  =  23.74

 

Conclusions:

 

     PTU mean is not significantly different from control (P > 0.05).

 

     Thyroxine mean is not significantly different from control

(although it was very close).

 

 

 

 

STATISTICAL ANALYSIS OF BODY WEIGHT DATA

 

 

TWO-WAY ANALYSIS OF VARIANCE

 

Source

df

Sum of 
Squares

Mean 
Square

F

P

Treatment

2

0.72

0.36

0.32

0.73

Weeks

1

10.96

10.96

9.55

< 0.01

Interaction

2

9.70

4.85

4.23

0.04

Error

12

13.77

1.15

Total

17

35.16

 

Conclusions:

 

There is no significant difference between initial treatment means.

 

There are highly significant differences between weeks.

 

There is a significant interaction between treatment and weeks.

 

     Thyroxine-treated rats lost weight (P < 0.05).

 

 

 

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Updated 2/14/08