MOLECULAR AND CELLULAR BIOLOGY LABORATORY
COURSE SYLLABUS
Spring 2007
Course
Number and Title: BIO 3101-001 Molecular
and Cellular Biology Laboratory
Laboratory:
Instructor: Gary A. Bulla, Ph.D.
Office: Life Sciences Building Rm 2027, Phone: 217-581-3499, Office hours:
Graduate
Teaching Assistant: Stephan Clark
Required laboratory
text: Must
can be purchased in Life Sciences
Annex Room 1120. Cost is $3.00.
Course Fee: $35.00
Course
Description:
Laboratory exercises are designed to provide on-hands experience with many
techniques used in molecular biology laboratories. Topics include DNA manipulation (including
recombinant techniques, construction of expression plasmids, restriction
mapping, subcloning, gel electrophoresis, chromosome mapping by PCR) as well as
protein analysis, RNA analysis, mammalian cell transfection, promoter analysis and
apoptosis.
Course Web
Site: www.ux1.eiu.edu/~gabulla
Desired Learner
Outcomes/Objectives:
1. Students will gain an
understanding of and experience in the basic principles of recombinant DNA
technology, including cloning, restriction analysis, PCR amplification, and
bacterial screening/selection techniques
2. Students will gain
experience in learn the proper use of controls in experimental design and
interpretation.
3. Students will learn techniques
used to isolate and assay detect specific components of DNA, RNA and protein.
4. Students will learn methods
to introduce DNA into mammalian cells and monitor expression of introduced
genes.
5. Students will understand
utility of the polymerase chain reaction (PCR).
Evaluation/Measurement/Assessment of Learner
Outcomes:
Exams.......................................................................................................................
30%
Exam I- 15%
Exam 2- 15%
Worksheets
. 20%
Weekly Quizzes
... 20%
Laboratory Reports...............................................................................................
20%
In Class activity.....................................................................................................
10%
Total 100%
Exams- Two laboratory exams worth
100pts. each will be administered during the semester. Exams will cover
material discussed in class and from laboratory exercises (both theory and
practical aspects).
Grading
Policy: Grades are derived from an
accumulated score of 100 percentage points:
A = 90 to 100 %, B = 80 to 89%, C = 70 to 79%,
D = 60 to 69%, and F = less than 60%.
Policy Statement on
Examinations: The times for the exams is
indicated on the course schedule. Exams
missed because of documented illness may be made up.
Disability Policy: To request academic accommodations due to a
disability, please contact me so that we can discuss the accommodations you
might need in class.
In-Class Activities: These
include attendance, cleanliness,
observations and activities assigned during laboratory time.
- Attendance
Policy: Laboratory attendance is mandatory. Laboratories require special reagents
and timing and thus are usually not
available at other times. Students
are expected to be punctual. The
use of cellular phones is not allowed in class.
- Lab
Cleanliness: You must clean up after
each experiment and be careful not to leave a mess around your lab area or
shared areas of the lab.
- Lab Observations and Activities: All labs have activities and experiments
you will be expected to perform and carefully observe. Several labs will require observation
and care of experiments outside of the lab period.
Laboratory Notebooks: Your lab notebook should contain everything
pertaining to the lab, including notes, drawings, data, speculations, and
methods. Your lab notebook is to be a
working notebook upon which you will base your lab reports.
Laboratory Reports and Worksheet
Assignments: For each laboratory
exercises, the student will prepare
laboratory worksheets at the conclusion of the experiments. Laboratory worksheets will be due at the beginning of the next lab period
(unless indicated otherwise in class). In
addition, lab reports will be required for some laboratory exercises (as
announced in class). Guidelines for preparing the lab reports and specific due
dates will be provided during the semester.
Lab reports and worksheets must be handed in on the due date to be
considered for full credit. If
the lab report or homework assignment is handed in late, it will be considered
for a reduced grade, i.e., if the report is late by one day (handed in Wednesday
by 4PM), it will be considered for the highest grade of B, if handed in Thursday
4 PM, it will be considered for the highest grade of C.
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SCHEDULE |
BIO 3101: Molecular and Cellular Biology Laboratory |
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Spring 2007 |
Location- LFSB Rm 2081 ( |
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Gary A. Bulla,
Ph.D. |
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Month |
Date |
Day |
Tentative Lecture Topic |
Lab # |
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Jan |
9 |
Tues |
Basic Techniques,
Bacterial Culturing |
1 |
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16 |
Tues |
DNA restriction and
electrophoresis |
2 |
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23 |
Tues |
Bacterial
transformation |
3 |
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30 |
Tues |
Mini-preps of DNA
and analysis Rm2010 |
4 |
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Feb |
6 |
Tues |
Recombinant cloning
(PART 1) |
5 |
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13 |
Tues |
Recombinant cloning
(PART 2) |
5 |
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20 |
Tues |
Polymerase chain
reaction |
6 |
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27 |
Tues |
Southern
hybridization-a1AT Exam
1 |
7 |
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Mar |
6 |
Tues |
Northern
hybridization- a1AT (PART 1) |
8 |
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13 |
Tues |
Spring Break |
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20 |
Tues |
Northern
hybridization - a1AT (PART 2) Lab write-up #1 |
8 |
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27 |
Tues |
Western analysis- a1AT (PART 1) |
9 |
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Apr |
3 |
Tues |
Western analysis- a1AT (PART 2) |
9 |
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10 |
Tues |
Mammalian
expression assay- Protein quantitation, b-gal
assay (ONPG) (PART 1) (in Rm2140) |
10 |
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17 |
Tues |
Mammalian
expression assay- Protein quantitation, b-gal
assay (ONPG) (PART 2) Lab Write-up #2 + Apoptosis (Day 1) |
10 +11 |
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24 |
Tues |
Apoptosis (Day 2) Exam 2 |
11 |
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Apr 30-May4 |
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Finals week |
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Important
Dates: |
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Deadline to Add Courses - Jan 12 |
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Deadline to Drop a Course with
No Grade- Jan 22 ( Deadline to Withdraw from a
Course or University with W- Mar 30 |
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